NGS molecular barcode script, Agilent Genomics NextGen Toolkit | Agilent
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AGeNT
RUO
The Agilent Genomics NextGen Toolkit (AGeNT) is a Java-based software module that processes the read sequences from targeted high-throughput sequencing data generated by sequencing Agilent SureSelect and HaloPlex libraries.
The Trimmer utility of the AGeNT module processes the read sequences to identify and remove the adaptor sequences and extracts dual molecular barcodes (for SureSelect XT HS2).
The LocatIT (for SureSelect QXT, XT LI and Haloplex HS) and CReaK (for SureSelect XT HS and XT HS 2) utilities of the AGeNT module process the Molecular Barcode (MBC) information from SureSelect and Haloplex HS Illumina sequencing runs with options to either mark or merge duplicate reads and output in BAM file format.
For Research Use Only. Not for use in diagnostic procedures.