Reverse Complement Sequence(s)

Reverse Complement Sequence(s)

The Reverse Complement Sequence(s) template lets you choose a source file and
choose the desired file output and location. Later, when you run the script, SeqNinja will create an output
sequence that is the reverse complement of the source file.

When you select this template, you will immediately be
prompted to select the source (input) file. Once you have done so, the File Type
of Reverse Complement wizard will ask you to choose the desired output file type
from a list.

Press Run to fill in the template based on your
selections and run the script immediately or Make to fill in the template
without running.

Choosing Make lets you review the information and make
any necessary changes before pressing Run:

•     
To change the source sequence, use the Choose Sequence(s) button
(see Add and Modify a
Sequence).

•     
To change the output format, make a selection from the Format
drop-down menu.

•     
To specify the name and location in which to save the output (see Specify Output Format
and Location) press the Save Results button and select a
location.

Note:
If you need to convert a file from .gbk to .fasta and back to
.gbk while preserving features that would normally be lost during
conversion to .fasta, prepend this command with SeqNinja’s “Set Full File
Conversion” step.

After setting up the template as
desired, click Run to run the script and create an output file. Or choose
Cancel to exit from the template without running the script.

Example:

LAMCG.seq begins with the string:
GGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAA.

Output file LAMCG_rev.fasta contains the reverse
complemented string, which ends with:
TTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC.